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Producing xylo-oligosaccharide from corncob xylan using xylanese from Indonesian Streptomyces isolates
Researcher : A. Meryandini, T. C. Sunarti
Published : 2006
Group :Microorganism
Full Report : Special request
Abstract :
Xylan is a second abundant polysaccharide in nature and major component of hemicellulose in the cell walls of land plants representing around 25-30% of total weight of its dry wood. Xylan is a component with high polydiversity such softwood-xylan consists most of arabino-4-o-metil glucuronat whereas hardwood-xylan consist most of o-asetyl 4-o-metil glucuronat. Softwood-xylan have a shorter structure compare to hardwood-xylan (Subramanian & Prema 2000, Beg et al. 2001). Because of the structure diversity of xylan, there is also diversity in xylanese, an enzyme that degrades xylan (Li et al. 2000).
Corncob is part from maiz which contents no more kernels, and is an abundant lignocellulosic raw material. To improve the nutritional value of this agricultural silage, we can use xylanese to produce xylosa, xylo-oligosaccharide (XOS) as dietary fiber or prebiotics compounds. For this research we will compare three acids xylanese from Indonesian Streptomyces spp that we have from previous research on 2 corn variety: Bisma and Hawai variety to produce dietary-fiber.
Streptomyces spp isolates were inoculated on 0,5% oat spelt xylan or 0,5%, 1% and 1,5% Bisma and Hawai xylan. The activity of xylanese was measured daily using oat spelt xylan. Several concentration of corn xylan and 0,5% oat spelt xylan was hydrolysed using xylanese crude extract for 5 hours on optimum temperature. Reducing sugar, total sugar and the degree of polymerization was calculated every hour and the composition of sugars is determined by TLC.
Xylooligosaccharides can be produced with the usage of strain 45I-3 xylanese in 1% corncob xylan in 4 hour of hydrolysis or with the usage of strain SKK1-8 xylanese in 0,5% corncob xylan in 3 hour of hydrolysis. Corncob xylan is less favorable for strain 234P-16 xylanese. This is signed by the higher of the degree of polymerization after 5 hours of incubation.