Update: 17 Apr 2020
Published: since 1 Mar 2008

Online Users: 2


Copyright © 2011
All Rights Reserved.

BIOTROP on FacebookFollow us on Facebook

us on Twitter

ISO 9001:2008 Certified

ISO 17025:2005 Certified

KNAPPP Certified
Rapid Detection of the Africanized Honey Bee : A Tool for Indonesian Animal Quarantine
Researcher : R. Raffiudin, A. Farajallah, B. Purwantara
Published : 2007
Group :Wildlife/Animal
Full Report : Special request
Abstract :
The spread of Africanized Honey Bee (AHB) through the continent started with the importation of honey bee of Apis mellifera. scutellata, an aggressive AHB. Since the introduction of an African subspecies into Brazil in the mid-1950's, descendents of 'Africanized' honey bees have spread throughout the Neotropics and into temperate North America. Indonesian Animal Quarantine provided no detection of this alien invasive species. Hence, this research was conducted to determine the rapid molecular detection of imported A. mellifera to Indonesia. DNA sequences from cytochrome b were explored to determine whether the Africanized honey bee occurred in the imported A. mellifera. The second objective was to examine the imported A. mellifera status in the Indonesian Animal Quarantine database. The result showed that out of 94 colonies samples from 82 keekeepers in Java, the same 485 bp PCR product was detected from the amplification of this gene. Two DNA fragments i.e. 194 and 291 bp was produced after digested with BglII. This cyt b/BglII result showed that all the collected samples of A. mellifera were the non-AHB type. Hence, there was no AHB in Indonesia. DNA extraction based on standard CTAB extraction and the DNA kit extraction produced the same result of 485 bp cyt b PCR product and the two DNA fragments i.e. 194 and 291 bp. Hence, the two types of DNA extraction kit from animal tissue could be used by the Animal Quarantine for AHB molecular rapid detection. Apis mellifera firstly entered Indonesia in 1972. Queen bees were imported to Indonesia until 1985 by Apiari Pramuka and other big beekeepers as recorded by the Agricultural Quarantine Agency. However, there was no records on the importation of queen bees at the Animal Quarantine Agency after the year 1985 due to no reports were submitted by the importers of beekeepers.