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Development of A Rapid Pcr-Based Detection Method of Black Leaf Streak Disease in Indonesian Banana Crops
Researcher : I. Hidayat, A. Hastuty
Published : 2010
Group :Biotechnology
Full Report : Special request
Abstract :
A study on development of a rapid PCR-based detection method of black leaf streak disease in Indonesian banana crops caused by M. fijiensis has been carried out. The main objecive of this study was to develop a fast, sensitive and specific PCR-based detection method for the presence of M. fijiensis in Indonesia. The methods include collection of specimens, morphological identification supported by molecular phylogenetic analysis, RFLP analysis, species-specific primers development, and validation. Two species of Mycosphaerella, namely, M. fijiensis and M. musicola, and one Pseudocercospora species (anamorphic state of Mycosphaerella) were found in Java island. Three restriction enzymes used in the RFLP analysis, viz, AluI, HaeIII, and TaqI were capable to discriminate M. eumusae, M. fijiensis, and M. musicola which are morphologically similar species. Two species-specific primer pairs, namely, MfijF/MfijR and MmusF/MmusR have been successfully developed to detect and to diagnose for the presence of M. fijiensis and M. musicola, respectively.

Key words: banana, detection, fungi, Mycosphaerella leaf spot, phytopathology, quarantine.